Taxonomic profiling of bacterial and archaeal communities from a CapScan-sampled gut metagenome

---

Subject: MD

Envivo Bio, Inc.; Confidential

1.1 Overview of microbial community composition

Figure 1 is an interactive figure showing the microbial community composition in the collected sample. Here, each color and shade corresponds to a different genus. For reference, domestic dogs (Canis familiaris) and gray wolves (Canis lupus) are different species that belong to the same genus (Canis).

You can interact with this figure by:

• Hovering the cursor over a color in the stacked bar to see the taxonomic identity, sample type, and relative abundance corresponding to that color and bar
• Clicking and dragging the cursor over a portion of the figure to zoom
• Single-clicking a genus’s name in the legend to remove that genus from the figure
• Double-clicking (very quickly) a genus’s name in the legend to only show that genus

Figure 1: Microbial community composition of collected CapScan sample. Taxonomic composition is reported at the genus rank. Genera present at <1% relative abundance across all samples are reported as “Other genera”. Note: Blue = Firmicutes; Yellow = Actinomycetes; Green = Bacteroidetes; Red = Proteobacteria; Purple = all other phyla. Shades of the same color (except purple) correspond to different genera from the same phylum.

1.2 High-resolution snapshot of community composition

Figure 2 is an interactive figure showing the microbial community composition at higher taxononomic resolution. Here, each row corresponds to a unique microbe.

You can interact with this figure by:

• Hovering the cursor over a cell to see that microbe’s genus and species (and strain when available), relative abundance, and full taxonomic classification
• Clicking and dragging the cursor over a portion of the figure to zoom

Figure 2: Heat map showing relative abundance of unique microbes (N = 103 total identified). Each row corresponds to a unique microbe. Note the relative abundance colorscale is in log units.

1.3 Notable microbes detected

• Faecalibacterium prausnitzii:
  • Previously shown to produce a small protein that alleviates gut inflammation in mouse models (Quévrain et al., 2017)
  • Found at ~1% relative abundance in distal CapScan sample
    
    
• Ruminococcus bromii:
  • Facilitates the breakdown of dietary starches that resist digestion (Ze et al., 2012)
  • Found at ~3% relative abundance in analyzed CapScan sample

1.4 References

Quévrain, E., Maubert, M.A., Michon, C., Chain, F., Marquant, R., Tailhades, J., et al. (2017) Identification of an anti-inflammatory protein from Faecalibacterium prausnitzii, a commensal bacterium deficient in Crohn’s disease. Gut 65: 415–425.

Ze, X., Duncan, S.H., Louis, P., and Flint, H.J. (2012) Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon. ISME J 6: 1535–1543.